Crystallization (Comment) | Organism |
---|---|
- |
Spinacia oleracea |
Protein Variants | Comment | Organism |
---|---|---|
E139K | site-directed mutagenesis, altered interaction and kinetics of enzyme-ferredoxin association compared to the wild-type enzyme | Anabaena sp. |
E301A | site-directed mutagenesis, altered interaction and kinetics of enzyme-ferredoxin association compared to the wild-type enzyme | Anabaena sp. |
K138E | site-directed mutagenesis, altered interaction and kinetics of enzyme-ferredoxin association compared to the wild-type enzyme | Anabaena sp. |
K290E | site-directed mutagenesis, altered interaction and kinetics of enzyme-ferredoxin association compared to the wild-type enzyme | Anabaena sp. |
K294E | site-directed mutagenesis, altered interaction and kinetics of enzyme-ferredoxin association compared to the wild-type enzyme | Anabaena sp. |
K72E | site-directed mutagenesis, altered interaction and kinetics of enzyme-ferredoxin association compared to the wild-type enzyme | Anabaena sp. |
K75E | site-directed mutagenesis, altered interaction and kinetics of enzyme-ferredoxin association compared to the wild-type enzyme | Anabaena sp. |
L76D | site-directed mutagenesis, altered interaction and kinetics of enzyme-ferredoxin association compared to the wild-type enzyme | Anabaena sp. |
L76F | site-directed mutagenesis, altered interaction and kinetics of enzyme-ferredoxin association compared to the wild-type enzyme | Anabaena sp. |
L76S | site-directed mutagenesis, altered interaction and kinetics of enzyme-ferredoxin association compared to the wild-type enzyme | Anabaena sp. |
L76V | site-directed mutagenesis, altered interaction and kinetics of enzyme-ferredoxin association compared to the wild-type enzyme | Anabaena sp. |
L78D | site-directed mutagenesis, altered interaction and kinetics of enzyme-ferredoxin association compared to the wild-type enzyme | Anabaena sp. |
L78F | site-directed mutagenesis, altered interaction and kinetics of enzyme-ferredoxin association compared to the wild-type enzyme | Anabaena sp. |
L78S | site-directed mutagenesis, altered interaction and kinetics of enzyme-ferredoxin association compared to the wild-type enzyme | Anabaena sp. |
L78V | site-directed mutagenesis, altered interaction and kinetics of enzyme-ferredoxin association compared to the wild-type enzyme | Anabaena sp. |
R100A | site-directed mutagenesis, altered interaction and kinetics of enzyme-ferredoxin association compared to the wild-type enzyme | Anabaena sp. |
R16E | site-directed mutagenesis, altered interaction and kinetics of enzyme-ferredoxin association compared to the wild-type enzyme | Anabaena sp. |
R264E | site-directed mutagenesis, altered interaction and kinetics of enzyme-ferredoxin association compared to the wild-type enzyme | Anabaena sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | associated to the grana fraction | Spinacia oleracea | 9507 | - |
membrane | weakly bound | Spinacia oleracea | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced ferredoxin + NADP+ | Anabaena sp. | delivers NADPH or reduced ferredoxin for several metabolic reactions, involved in photosynthesis | oxidized ferredoxin + NADPH | - |
r | |
reduced ferredoxin + NADP+ | Spinacia oleracea | delivers NADPH or reduced ferredoxin for several metabolic reactions, involved in photosynthesis, enzyme-substrate interactions, overview | oxidized ferredoxin + NADPH | - |
r | |
reduced ferredoxin + NADP+ | Escherichia coli | reverse reaction is involved in activation of enzymes that participate in anaerobic metabolism | oxidized ferredoxin + NADPH | - |
r | |
reduced ferredoxin + NADP+ | Azotobacter vinelandii | reverse reaction is involved in activation of enzymes that participate in anaerobic metabolism, removal of free radicals gegnerated during the metabolsim | oxidized ferredoxin + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anabaena sp. | - |
- |
- |
Azotobacter vinelandii | - |
- |
- |
Escherichia coli | - |
- |
- |
Spinacia oleracea | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH | reaction mechanism via semiquinone intermediate and radical formation | Escherichia coli | |
2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH | reaction mechanism via semiquinone intermediate and radical formation | Azotobacter vinelandii | |
2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH | reaction mechanism via semiquinone intermediate and radical formation, enzyme-substrate interactions, overview | Spinacia oleracea | |
2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH | reaction mechanism via semiquinone intermediate and radical formation, enzyme-substrate interactions, overview | Anabaena sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is asscoiated to phycobilin pigments | Anabaena sp. | ? | - |
? | |
reduced ferredoxin + NADP+ | delivers NADPH or reduced ferredoxin for several metabolic reactions, involved in photosynthesis | Anabaena sp. | oxidized ferredoxin + NADPH | - |
r | |
reduced ferredoxin + NADP+ | delivers NADPH or reduced ferredoxin for several metabolic reactions, involved in photosynthesis, enzyme-substrate interactions, overview | Spinacia oleracea | oxidized ferredoxin + NADPH | - |
r | |
reduced ferredoxin + NADP+ | reverse reaction is involved in activation of enzymes that participate in anaerobic metabolism | Escherichia coli | oxidized ferredoxin + NADPH | - |
r | |
reduced ferredoxin + NADP+ | reverse reaction is involved in activation of enzymes that participate in anaerobic metabolism, removal of free radicals gegnerated during the metabolsim | Azotobacter vinelandii | oxidized ferredoxin + NADPH | - |
r | |
reduced ferredoxin + NADP+ | plant-type ferredoxin contains a [2Fe2S] cluster, enzyme requires ferredoxin, substrate is a bulky protein, enzyme-substrate interactions involve residues R16, K72, K88, K116, E139, R264, K290, and K294, overview | Spinacia oleracea | oxidized ferredoxin + NADPH | - |
r | |
reduced ferredoxin + NADP+ | plant-type ferredoxin contains a [2Fe2S] cluster, substrate is an acidic, bulky protein, enzyme-substrate interactions involve residues R16, K72, K75, R100, E139, R264, K290, and K294 | Anabaena sp. | oxidized ferredoxin + NADPH | - |
r | |
reduced ferredoxin + NADP+ | substrate is a bulky protein | Escherichia coli | oxidized ferredoxin + NADPH | - |
r | |
reduced ferredoxin + NADP+ | substrate is a bulky protein | Azotobacter vinelandii | oxidized ferredoxin + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional structure analysis, intramolecular stabilization and stabilizationof enzyme-substrate complex, overview | Anabaena sp. |
More | three-dimensional structure analysis, overview | Escherichia coli |
More | three-dimensional structure analysis, overview | Spinacia oleracea |
More | three-dimensional structure analysis, overview | Azotobacter vinelandii |
Synonyms | Comment | Organism |
---|---|---|
FNR | - |
Escherichia coli |
FNR | - |
Spinacia oleracea |
FNR | - |
Azotobacter vinelandii |
FNR | - |
Anabaena sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | involved in reaction splitting a two-electron-reaction into 2 one-electron-reactions | Escherichia coli | |
FAD | involved in reaction splitting a two-electron-reaction into 2 one-electron-reactions | Spinacia oleracea | |
FAD | involved in reaction splitting a two-electron-reaction into 2 one-electron-reactions | Azotobacter vinelandii | |
FAD | involved in reaction splitting a two-electron-reaction into 2 one-electron-reactions | Anabaena sp. | |
NADP+ | - |
Escherichia coli | |
NADP+ | - |
Azotobacter vinelandii | |
NADP+ | interaction with the enzyme, complex structure, specificity-determining structures, overview | Spinacia oleracea | |
NADP+ | interaction with the enzyme, complex structure, specificity-determining structures, overview | Anabaena sp. |